Comparative studies on updated isolates of Foot and mouth disease virus in cattle in kafr El Sheikh governorates
Abstract: Foot and mouth disease virus (FMDV) has been annually isolated during the recent years in Egypt. There is no sufficient local data about the genetic changes occurred in the virus, which makes tracing of its oritgin difficult. The characteristics of the FMDV that caused sporadic cases in Kafr El-Sheikh, Egypt in late 2010 have not yet been reported . Suspected FMD virus samples, Tongue epithelium (TE) and vesicular fluid (VF) were isolated on cell-culture monolayer of baby Hamster Kidney (BHK-21) cells and suckling baby mice then serotype by complement fixation test (CFT). Direct fluorescent antibody technique (FAT) and agar gel immune diffusion test (AGIDT) used as confirmatory tests. Cell culture grown virus that was identified as serotype O "FMD virus by complement fixation test. It was found that all virus isolates shared a closer relationships to the current strain with r-value" ranged between were 0.86-0.88 by using virus neutralization test.
In addition, molecular detection, nucleotide sequencing and phylogenetic analysis of the obtained FMDV isolates are performed to study the molecular epidemiology of the virus. Changes in the sequence of the VP1 coding region of these viruses were identified. RNA extracted from either clinical or cell culture grown virus was subjected to Real time – Polymerase chain reaction RT-PCR and specific amplified DNA segments for FMD were amplified. Nucleotide sequencing of FMDV VP1 gene was determined. The phylogenic analysis revealed that the isolated FMDV virus in Kafr El- Sheikh Governorates 2010 was homologous with O/Sharquia 72 and the used vaccinal strain O1/3/Aga93.
Publication year 2012
Pages 54-63
Organization Name
City كفر الشيخ
serial title veterinary journal
ISSN 1110-1458
Author(s) from ARC
External authors (outside ARC)
    سامي قاسم قسم الفيرولوجي كلية طب البيطري
Agris Categories Animal diseases
Publication Type Journal
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