Study on stability and immunogenicity of using inactivated freeze dried AI virus after reconstitution in immune stimulatory adjuvant
Abstract: Preliminary study was done to spot light on the effect of lyophilization (freeze-drying process) on stability and immunogenicity of inactivated avian influenza (AI) virus using two different stabilizer-skimmed milk and lactose peptone buffer. The lyophilized inactivated virus was tested with two different adjuvants for reconstitution (DEAE-Dextran and Mantonide oil. The best immune response among vaccinated birds groups as measured by Haemagglutination inhibition test (HI) achived by using lactose peptone buffer as virus stabilizer and DEAE-Dextran as adjuvant, followed by the group vaccinated with freeze dried inactivated virus with the same stabilizer coordinated with Mantonide oil as adjuvant. When skimmed milk was used as virus stabilizer, results were acceptable. Stability of the two selected batches that were serological proved to be the most successful revealed that the virus antigen was having an immunogenic effect for six month when kept at -20°C or 4°C when reconstituted and inoculated in susceptible chicken.
Publication year 2011
Pages 11-16
Organization Name
City zagazig
serial title zagazig veterinary journal
ISSN 1110-1458
Author(s) from ARC
Agris Categories Animal diseases
Publication Type Journal
↑