Trials for preparation of monovalent inactivated equine influenza vaccine
Abstract: Equine influenza virus ( A/equi-2/ Cairo-2/2000, H3N8) with egg passage 6 (Ep6) was inactivated by 0.1% formalin (40% formaldehyde) and complete inactivation was achieved after 24 hours at 37o C.
two forms of monovalent equine influenza vaccine were prepared using different adjuvants, the first with DEAE- Dextran, while the other with alhydragel and saponin. Both vaccine forms (freeze dried and liquid one) proved to be safe and potent for horses and G. pigs. The mean HI antibody titres in G. Pigs( three weeks post inoculation) were 1408, 1344, while in horses( six weeks post inoculation) were 640, 480 respectively for both vaccines.
The antibody titre in horses vaccinated with both vaccine forms were monitored and persisted at protective level for one year.
The keeping quality of the locally prepared EI monovalent inactivated vaccine either freeze- dried, reconstituted in DEAE- Dextran or the liquid one were studied and the shelf validity for both vaccines were found to be stable at 4o C for one year, while the freeze dried one can be kept at -20oC for three years
Publication year 2009
Organization Name
City بنها
serial title المؤتمر العلمي الثالث لكلية الطب البيطري -جامعة بنها
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Dextrans. Equine influenzavirus. Rabbits. Vaccines.
Publication Type Conference/Workshop
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