COMPARATIVE EVALUATION OF THE POTENCY OF TRADITIONALLY INACTIVATED BOVINE EPHEMERAL FEVER VACCINE AND THAT ONE INACTIVATED ON THE TIME OF VACCINATION

Abstract: This study determined the usefuless of saponin as adjuvant additive to the diluents of live bovine ephemeral fever vaccine (BEF). Firstly the cytotoxicity and antiviral effect of saponin were investigated on BHK-21 cells and against BEF virus respectively where it was found that concentration 0.1 and 0.2 µg/ml of saponin did not induce cytotoicity in BHK-21 cells while an antiviral effect was noticed against 100TCID50 of BEF virus. Saponin showed its antiviral effect against BEF virus when it was inoculated either before or simultaneously with of after one hours of virus inoculation. Addition of 0.2 µg of saponin/ dose of live BEF vaccine inactivate the virus and induced higher antibody titers by the first week post the first vaccination than that induced by the gel vaccine alone. Such antibody levels still higher with saponin than the gel vaccine up to 4 months 2nd booster dose suggesting the performance of long duration of immunity. So it could be recommended to add saponin to live BEF vaccine as inactivator on the time of vaccination providing safe and highly potent vaccine.
Publication year 2010
Pages 51-52
Organization Name
City أسيوط
serial title المؤتمر العلمي الرابع عشر
Author(s) from ARC
External authors (outside ARC)
    نرمين جودة شفيق المعمل المركزي للرقابة عليالمستحضرات الحيوية
    محمد أحمد سعد المعمل المركزي للرقابة عليالمستحضرات الحيوية
    أحمد محمد البهوار المعمل المركزي للرقابة عليالمستحضرات الحيوية
    ماجد منير ابراهيم المعمل المركزي للرقابة عليالمستحضرات الحيوية
    ماجدة سيد محمد المعمل المركزي للرقابة عليالمستحضرات الحيوية
    أنهار عبد المعطي المعمل المركزي للرقابة عليالمستحضرات الحيوية
Publication Type Conference/Workshop
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