Pathogenesis Of African Horse Sickness Virus In Dogs
Abstract: two susceptible foals were inoculated I/V with 2 and 10 cc of viscerotrotropic AHSV type 9 strain (6/63) virulent horse blood ) respectively.
2- the blood was collected from the jugular vein of the second foal on the 4th and 5th day P.I . when the recorded temperatures were 39.5 oC and 40. 0 oC respectively.
3-the defibrinated horse blood hand a titer log10 6.6 MLD 50 ml .when titrated in suckling mice.
4- the spleen , lung, heart and kidney of the 2nd foal were collected on hank’s solution and kept at -20 oC .
5- 28 susceptible balady dog of 4-5 months old were classified into 3 groups, each group of 8 dogs and 4 dogs were left as uninfected controls.
6- the groups of dogs were kept separately in insect proof kennels and their temperature recorded twice daily.
7- each dog of the first group was fed infected horse viscera and virulent horse blood after an overnight starvation together with S/C injection of each dog with 5 ml . virulent defibrinated horse blood .
8- each animal of the second group of dogs was infected orally with horse viscera and virulent horse blood after an overnight starvation.
9- the third group of dogs were subcutaneously inoculated with 5 ml .virulent defibrinated horse blood for each dog.
10- one dog from each group of the experimentally infected dogs was sacrified on the 4th ,5th , 10th , 20th , and 45th dog post – infection according to the febrile reaction of the dogs .
11- the remaining 9 dogs were kept till 90 days P .I. for obtaining sera to study the seroconversion of infected dogs.
12- AHSV was isolated from blood samples of dogs sacrified on the 4th , 5th ,and from one blood sample collected on the 10th , day P .I .
13- the highest titer of the reisolated virus in suckling mice was log10 5 MLD50 / ml . (on the 4th days P .I.) and the lowest titer was log10 < 1 MLD50 / ml . (on the 10th day post –infection).
14- isolation of AHSV from both dog viscera and dog excreta in suckling mice for three successive passages failed.
15- the complement fixing antibodies appeared in the sera of experimentally infected dogs after 10 days P. I . with a titer of 16 and reached the peak at 20 days P.I. with a titer of 32.
16 – the neutralizing antibodies appeared in the sera of experimentally infected dogs after 20 days P. I. with a titer of 16 and reached the peak at 75 days P.I. with a titer of 128.
17- no precipitating antbodies could be detected in the sera of all experimentally infected dogs the observation period (3 months).
African horse sickness viral antigen was present in all visceral organs of experimentally infected dogs and could be detected by indirect fluorescent antibody technique and agar gel precipitation test .
19- presence of AHS viral antigen by indirect FAT in the spleen and lungs was 83.3% and 66.7 % respectively.
20- presence of AHS viral antigen by AGPT in the spleen and lungs was 55.6% and 44.4% respectively.
Publication year 1991
Availability location معهد بحوث الامصال واللقاحات البيطرية-ش السكة البيضاء- العباسية - القاهرة
Availability number
Organization Name
Country Egypt
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 African horse sickness. Dogs. Pathogenesis. Viroses.
Publication Type Master Thesis
↑