Some Studies on Attenuated Rift Valley Fever Vaccine
Abstract: RVF is an infectious disease. The best mean to control the disease was vaccination and eradication of vector. Many vaccines were used for this purpose, one of them it was mutagen variant using 5 - flurouracil on tissue culture. In this study trials were conducted to prepare attenuated vaccine using 5-flurouracil.
Firstly, titration of original RVF strain was conducted on BHK and CER cell line, the titer was 7.5 and 7.8 log10 TCID50/0.1 ml respectively also titration using adult mice resulting in titer of 10 6.5 MIPLD50/0.1ml. The safe concentration of 5-flurouracil on BHK cell and in adult mice were 100-300 ug/ml, but concentration over 300 ug/ml were toxic for both tissue culture and adult mice. The effect of 5 – flurouracil on RVFV was studied using passages in adult mice after treatment with different concentration of 5- flurouracil (100, 200 and 300ug/ml) virus titer in adult mice and pathogenicity in baby mice were conducted to every serial passages. The titer was decreased from 106.5 MIPLD50 /0.1 ml to under one log 10 MIPLD50 /0.1 ml after using 300 ug/ml concentration of 5-flurouracil. The pathogenicity of this passage was lowered as the adult mice inoculated with it were survived for 3 weeks after inoculation, but it caused 70 % deaths among baby mice. This passaged virus was adapted for two passages on BHK cells and was titrated using suckling mice, its titer was 7.4 and 7.3 log10 MICLD50 /0.02 ml. This adapted virus (MP17) was tested by further 7 passages in suckling mice without addition of 5 – flurouracil with MP17 and titrated. The titer was ranged from 7.0 to 7.2 log 10 MICLD50 / 0.02ml. MP17 was exposed to potency test by inoculating adult mice resulting in 90-100 % protection. Mp17 virus was evaluated in pregnant mice. It did not abortogenic and the delivered baby mice were normal and healthy .
Further evaluation was conducted in adult sheep by inoculation of one group (4 sheep) with 1 dose (1 ml of 104 MICLD50/0.02 ml mp17 virus. Seroconversion studies were studied using SNT and ELISA tests. After one month these animals were challenged with virulent RVFV. Vaccinated animals with MP17 did not show any reaction post challenge with virulent RVFV.
Publication year 1994
Pages 79-80
Availability location معهد بحوث الامصال واللقاحات البيطرية
Availability number
Organization Name
Country Egypt
Publisher Name: كلية الطب البيطري بمشتهر - الزقازيق فرع بنها
Volume 1 . 1
Author(s) from ARC
External authors (outside ARC)
    محمد حسنين عبيد استاذ الأمراض المعدية - كلية الطب البيطري - جامعة الزقازيق
    محمد محمود طه رئيس بحوث بقسم حمى الوادي المتصدع - معهد بحوث الأمصال واللقاحات
Agris Categories Animal diseases
Proposed Agrovoc Rift Valley Fever Vaccine;
Publication Type Master Thesis
↑