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Immungenicity of some protein fractions of mycobacteria
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Abstract: Purification of 45/47 Da protein complex from culture filtrate of BCG by Si200 column, six major fractions collected, 45/47 Da present in fraction 2, protein content was 1 mg/ml whole culture filtrate of M. bovis prepared by SDS-Page analysis there were band of 45/47 Da culture filtrates ( M.Bovis, BCG ) harvested after 14 days. Vaccination of guinea pigs by 45/47 kda, BCG culture filtrates ( M.Bovis, BCG ) challenged after 12 weeks. vaccines were evaluated by humoral immune response (indirect ELISA), cell mediated immune response (lymphocyte proliferation assay expressed by MTT). studies of protective immunity : survival rate, root specific lung weight (RSLW). Histological appearance postchallenge by 28 days. Protection against tubercles infection cannot be guaranteed by 45/47 da alone
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Publication year |
2000
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Availability location |
معهد بحوث الامصال واللقاحات البيطرية
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Availability number |
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Organization Name |
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Country |
Egypt
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External authors (outside ARC) |
صلاح الدين عبد الكريم سليم
كلية طب بيطرى-جامعة القاهرة
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Agris Categories |
Animal diseases
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AGROVOC TERMS |
Proteins.
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Proposed Agrovoc |
مناعية بعض الأجزاء البروتينية;ميكروب السل البقري;
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Publication Type |
PhD Thesis
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