Comparative Studies on Brucella Antigen Prepared By Different Methods

Abstract: This work was a trial to prepare new vaccine that can stimulate the immune response to protect cattle, sheep, goats and all other ruminants against both Brucella abortus and Brucella melitensis.
Local strain of Brucella melitensis biovar 3 (which bears both A and M antigen in nearly equal amount) was mutagenized chemically using Nitro – Nitroso- guanidine mutagen.
All strains used in the experiments were biochemically identified.
For estimating of the virulence of all strains used, groups of guinea pigs were used. Groups were inoculated with 5x 103 cfu (one group for each strain) groups inoculated with brucella abortus were scarified after 8 weeks of injection and groups with Brucella melitensis were sacrified after 12 weeks.
Spleens of all animals were removed and weight aseptically, ratio of spleen / body weight were calculated ; colony forming /gm spleen were counted virulence of each strain were discussed.
Vaccines were prepared from : Brucella melitensis biovar 3 mutant in 2 doses a high one of 7.5 x 109 cfu and a low dose of 1.5 x 10 9 cfu, Brucella abortus strain 19 with a dose of 7.5 x 10.9 cfu and brucella melitensis strain Rev 1 with a dose of 1.5 x 10.9 cfu.
For estimating the potency of each vaccine, groups of guinea pigs were vaccinated with brucella abortus strain 19 or biovar 3 mutant high dose and other groups were vaccinated with brucella melitensis strain Rev 1 or biovar 3 low dose.
All vaccinated groups were challenged with homologous virulent strains. Animals were sacrified ; spleen count and ratio of spleen/body weight were calculated.
All animals vaccinated and challenged were bled weekly from the beginning of inoculation till the end of the experiments.
Publication year 1994
Availability location ش السكة البيضاء - العباسية-القاهرة
Availability number
Organization Name
Country Egypt
Volume 1 .
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Antigens. Brucella.
Publication Type PhD Thesis
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