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تطوير نظام تجديد في المختبر من النورات غير الناضجه وتعديل الجينات بواسطة كريسبر/كاس9 CRISPR/Cas9 في الذره الرفيعه
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الملخص: Background Sudangrass (Sorghum sudanense) is a major biomass producer for livestock feed and biofuel in many countries. It has a wide range of adaptations for growing on marginal lands under biotic and abiotic stresses. The immature inflorescence is an explant with high embryogenic competence and is frequently used to regenerate different sorghum cultivars. Caffeic acid O-methyl transferase (COMT) is a key enzyme in the lignin biosynthesis pathway, which limits ruminant digestion of forage cell walls and is a crucial barrier in the conversion of plant biomass to bioethanol. Genome editing by CRISPR/Cas9-mediated mutagenesis without a transgenic footprint will accelerate the improvement and facilitate regulatory approval and commercialization of biotech crops.
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سنة النشر |
2023
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أسم المركز |
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اسم الجريدة |
Journal of Genetic Engineering and Biotechnology
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مؤلفين من مركز البحوث الزراعية |
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مؤلفين من خارج مركز البحوث الزراعية |
M. H. Abd El-Aziz
Genetics Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt
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نوع المنشور |
دورية
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