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se of monospecific antisera for differential diagnosis of classic and
variant strains of infectious bronchitis virus
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Abstract: This study was carried out to investigate the efficacy of mono-specific antisera prepared in rabbits for the detection and
differential diagnosis of classic and variant strains of infectious bronchitis virus (IBV) in comparison with those prepared in
specific pathogen free (SPF) chickens in addition to their ability in detection of the degree of cross neutralization and antigenic
relationship between these strains. Two serological tests were used; indirect haemagglutination inhibition test (IHI) and serum
neutralization test (SNT). Also, two classic (H120, MA5) and two variant (4/91, CR88) IB antigens were used. IHI test gave
higher titers not only in case of homologous strains than heterologous but also higher in rabbits than chickens (H120 strain Vs
H120 antisera 12 log2 in rabbits and 9.6 log2 in chickens. The results of IHI were confirmed by using SNT that classic (H120) and
variant (4/91) strains gave high neutralizing index (NI) when using antisera for rabbit (0.5) while in chickens H120 and CR88
gave (3.8). So, rabbit is recommended animal for preparation of commercial monospecific hyperimmune sera which can be
successfully used in detection and differentiation of classic and variant strains of IBV and evaluation of antigenic relationship
between these strains.
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| Publication year |
2015
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| Pages |
58–65
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| Organization Name |
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| Author(s) from ARC |
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| Publication Type |
Journal
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