Effect of bacterial inoculation and spraying with K2HPO4 on egyptian clover in a calcareous soil
Abstract: Two field experiments were conducted in a normal calcareous soil at Noubaria to recognize the benefits of inoculation with some N2-fixing bacteria and /or two ascending doses of K2HPO4 on improvement of Egyptian clover (Trifolium alexandrinum) growth.
In the both first and second trials, seeds were inoculated with Rhizobium leguminosarum biovar trifolii on sowing day. Then, plants were sprayed three times with some N2-fixing bacteria, Bacillus polymyxa, Azospirillum brasilense and Azotobacter chroococcum.. Plants were sprayed three times with two rates of K2HPO4, the first 0.8 Kg P and 2 Kg K/fed as recommended rate and the second was twice of the recommended one. Three cuts were taken after 11, 20 and 30 weeks of planting in the 1st. season and two cuts were taken after 11, 30 weeks of planting the 2nd.one
The obtained data showed that inoculation with N fixing micro-organisms to increase fresh and dry yield of clover significantly over the non inoculated plant. Uptake of N, P and K in all the cuts increased significantly with that treatment which also activated nitrogenase in root nodules and dehydrogenase in rhizosphere soil over the significancy limits.
Foliar application of K2HPO4 followed almost the same trend of inoculation in case of yields, N, P and K uptake regardless the used rate of application in most cases and thus the low rate of application (0.8 kg P and 2 kg K/fed) was sufficient to correct the P, K deficient in such soil.
. The combination of the two factors inoculation and K2HPO4 application resulted in some interaction effects on those studied parameters were discussed and in the other cases, each of the two factors played its role individually.
Finally, inoculation clover seeds with N¬2¬¬-fixing bacteria and foliating clover plants with 4.5 kg K2HPO4 arranged on 3 equal times may be recommended to obtain the best quantity and quality of clover under such soil condition in addition to the residual effect on soil. Di potassium phosphate did not activate significantly nitrogenase and dehydrogenase enzyme
Publication year 2005
Availability location Soils, Water and Environment Research Institute, ARC
Availability number
Organization Name
Country Egypt
City Cairo
Publisher Name: The National Center for Information & Documentation (NIDOC)
serial title Egyptian Journal of Soil Science
ISSN 0302-6701
Volume 45 .
Department Agricultural Microbiology Research
Author(s) from ARC
Agris Categories Soil fertility
AGROVOC
TERMS		 Azospirillum brasilense. Azotobacter. Calcareous soils. Nitrogenase. Oxidoreductases. Phosphate fertilizers. Rhizobium leguminosarum. Trifolium alexandrinum.
Publication Type Journal
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