Molecular diagnosis of bovine mycoplasma mastitis
Abstract: SUMMARY:
Fifty-eight milk samples collected from private farms, (in which mycoplasma infection was suspected) were obtained for mycoplasma species and Mycoplasma bovis detection. All samples were examined by culture, polymerase chain reaction (PCR) and dot blot hybridization techniques. The results indicated that mycoplasma species were detected from 20. 30 and 30 milk samples by culture, PCR and dot blot hybridization techniques, respectively, while M. bovis was detected from 14, 26 and 24 samples, respectively. The revised sensitivities of detecting the mycoplasma species by culture, PCR and dot blot hybridization techniques were 66.7%, 100% and 100%, respectively, while the sensitivities of detecting the M. bovis were 58.3 %, 100%, 100%, respectively. On the other hand, the revised specificities were 100% for detection of mycoplasma species by culture, PCR and dot blot hybridization techniques, while the revised specificities of detecting the M. bovis were 100%, 94.1% and 100%, respectively. The results also indicated that all negative control samples (22) were negative by all tested techniques.
The results indicated that, PCR and dot blot hybridization techniques are reliable, rapid, highly sensitive, and specific for accurate detection of mycoplasma species as well as M. bovis in milk samples.

Key words: Mycoplasma species, M. bovis, milk, PCR, dot blot hybridization
Publication year 2004
Availability location مكتبة معهد بحوث التناسليات الحيوانية
Availability number
Organization Name
Author(s) from ARC
Agris Categories Veterinary science and hygiene - General aspects
Proposed Agrovoc Mycoplasma species;
Publication Type Researsh & Applied Activities
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