Aclinico-Pathological Studies on the Relationship between Some Blood Constituents and Endometrial Biopsies Associated with Some Reproductive Disorders in Buffaloes
Abstract: ABSTRACT:
The present study was carried out to investigate the changes in blood serum constituents as well as histopathological alterations in the endometrial Biopsy associated with some causes producing infertility in Egyptian buffaloes. A total of 55 buffalo-cows from El-Ibrahemia- Sharkia veterinary clinics were studied. Based on the case history and rectal as well as vaginale xaminations, the animals were classified into 4 groups according to their reproductive performance: the 1—group of (n=12) involved normally cycling buffaloes attended to the clinic in follicular phase (6-animals) or luteal )phases (6-animals) of the estrous cycle which was confirmed by the rectal examination. The animals in the follicular and luteal phases are considered [the control group). The 2— group of (n=21) buffaloes had a pathological affection in their uteri. The animals had a history of repeat breeder and the rectal palpation revealed the presence of endometeritis with turbid vaginal discharge. The 3~ group of (n=16) buffaloes had inactive ovaries. There was a history of post partum anaestrum for about 6 months. The 4~ group of (n=6) buffaloes had follicular cyst on their ovaries. The cyst was markedly protruded fluctuating vesicular structures without functional corpus luteum on the ovarian surface. Blood samples for serum separation were taken from normal and infertile buffaloes for determination of total proteins, albumen, globulins, globulin fractions and hormonal assay (Estradiol-17β, progesterone and Cortisol) as well as endometrial biopsies for histopathological examinations. The obtained results demonstrated that, buffaloes suffering from endometeritis showed significant elevation in the total proteins, globulins, gamma- globulins, Cortisol hormone and significant decrease in estradiol and progesterone values, with severely infiltration of the endometrium by inflammatory cells mostly of mononuclear type. Buffaloes suffering from ovarian inactivity showed significant hypoproteinaemia, hypoglobulinaemia, hypogamma¬globulinemia with highly significant decrease in estradiol and progesterone profile and the blood vessels of the endometrium had a thick wall with narrow lumen. Meanwhile, buffaloes affected with cystic ovaries had a highly singnificant elevation in their hormonal profile with no signinficant change in their serum biochemical constituents and some of the endometrial glands showed degenerative and atrophieed chaanges, incomparison to the normal cyclic animals.It can be concluded that the correlation between clinical estimation of some blood parameters and histopathological study of the endometrial biopsy is necessary for complete diagnosis of infertilty problems among egyptian buffaloes.
Publication year 2006
Pages 253-270
Availability location معهد بحوث التناسليات الحيوانية -الهرم
Availability number
Organization Name
Author(s) from ARC
Agris Categories Animal physiology - Reproduction
Publication Type Researsh & Applied Activities
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