Buffalo anestrum: diagnosis and treatment
Abstract: Anestrus is the main factor that negatively affects reproductive performance of domestic animals bred of the globe. In Egypt, anestrus remains an important problem for reproduction in buffalo. This study aimed to diagnose some causes of anestrus using ultrasonographic examination as an accurate method for diagnosis of ovarian structures and more reliable than rectal palpation for diagnosing follicles as well as Corpus luteum as well as to investigate the effect of PGF2(X GnRH and Bovine somatotropin (BST) on treatment of anestrus in Egyptian buffalo-cows and subsequent on reproductive performance. 42 anestrus buffalo-cows calved 3-11 months ago were used in the present study. Ultrasonographic examination of the genital organs (ovaries, uterus, and cervix) and progesterone levels revealed that these animals had either Corpus luteum 12 (28.57%) or inactive ovaries 30 (71.43%) with normal genital tract. Buffalo-cows with Corpus luteum (group I) were treated by 3 ml estrumate (synthetic PGF^a). Buffalo-cows with inactive ovaries (group II) were divided into 2 subgroups. The fust subgroup Gil A (n=l5) was treated by subcutaneous administration of 500 mg of bovine somatotropin followed by intramuscular injection of 5 ml receptal (GnRH analogue) 5 days later. The second subgroup Gil B(n = 15) was treated with intramuscular injection of 10 ml receptal divided into 2 doses, 5 ml every 24 hours followed by injection of 3 ml estrumate 7 days later. All animals were observed for estrus detection twice daily (at down and evening) and those came in estrus were mated. Serum progesterone concentrations were measured to determine ovarian activity prior to treatment and to confirm the occurrence of ovulation as well us to determine early pregnancies which were confirmed by ultrasonographic examination 30 to 40 days post sen>ice.
The results of the present study indicated that ultrasonography is a reliable method for diagnosing the functional stale of the ovary in hujfalo-cows. Treatment of ovarian inactivity in buJJ'alo-cows using GnRH with BST or GnRH with PGFjCC gave good fertility as Indicated by short treatment estrus interval (5.47 + 0.8 and 6.13 + 0.7 days, respectively) and high first service conception rate (53.3%) as well as overall conception (73.3%) rate. Treatment of animals carrying corpora lutea on their ovaries by using PGF2agave shorter time (4.2 ± 1.3 days) to the first estrus but with lower first service conception rale (41.6%) and over all conception rate (50%).

Key words: Buffalo-Anestrum, GnRH, PGF2a, bovine somatotropin. Reproductive
Performance, Ultrasound, Ovaries.
Publication year 2005
Availability location معهد التناسليات الحيوانية بالهرم
Availability number
Organization Name
Author(s) from ARC
External authors (outside ARC)
    بسيونى احمد هليل كلية طب بيطرى -كفر الشيخ
    اسماعيل القن كلية طب بيطرى -كفر الشيخ
    سامى احمد درويش معهد الانتاج الحيوانى بكفر الشخ
Agris Categories Veterinary science and hygiene - General aspects
Proposed Agrovoc Buffalo-Anestrum, GnRH, PGF2a,;
Publication Type Researsh & Applied Activities
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