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Trials for preparation of clostridium tetani toxoid by using different chemicals
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Abstract: The use of different chemical in activators for modification of Cl.tetani toxin was studied. Formalin is still used until now as a good toxoiding agents. The attention is directed to the study of other detoxifying agents due to some objections of using formalin it causes destruction of some of the toxin antigens, if added in high doses, formalin is an excellent cross linking agent with the impurities in teh medium and it needs 3-4 weeks incubation to complete toxoiding. Different concentrations of Binary ethylenimine (BEI)were tried in this study. The toxin treated BEI was compared with the formalized toxin by flocculation test, challenge test, ELISA technique SDS page and irreversibility. The obtained results revealed that the concentration 0.08BEI was the best concentration that gave complete toxoiding in a short period(3days)with no reversibility, and kept the antigenicity of the toxoid.
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| Publication year |
2005
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| Pages |
1
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| Availability location |
معهد بحوث الامصال واللقاحات البيطرية القاهرة - ش السكة البيضاء - العباسية ص ب 131
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| Availability number |
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| Organization Name |
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| City |
الزقازيق
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| serial title |
مجلة الزقازيق البيطرية
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| ISSN |
1110-1458
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| Department |
Equine Vaccines Research
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Elisa.
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| Proposed Agrovoc |
Clostridial tetani;
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| Publication Type |
Journal
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