Molecular detection of babesia equi in infected and carrier horses by polymerase chain reaction
Abstract: Twenty three blood samples were used in this study; five were from five naturally infected horses with Babesia equi ( B. equi), while eighteen were from asymptomatic horese with equine babesiasis from different localilties in Egypt. All samples were subjected to microscopic examination, indirect fluoerscent antibody test (IFA) and polymerase chain reaction (PCR) The carrier animals were microscopically detected in 7 out of 18 samples (38.8 % ) and in 9 of 18 by using IFA (50%) whereas PCR revealed that 14 samples were positive (78%) two synthetic oilgonucleotide primers, based on the B . equi merozoite antigen gene (EMA-1) were used. A.819 bps DNA fragment is specifically amplified from the gene encoding EMA-1 of B. equi. Our results demonstrate that PCR is a valuable technique for routine detection of B. equi in chronically infected horses, even at low parasitaemia levels .
Publication year 2003
Availability location معهد بحوث الامصال و اللقاحات البيطرية - القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City القاهرة
serial title The Egyptian Journal of Immunology
Department Bacteriological Diagnostic Production Research
Author(s) from ARC
External authors (outside ARC)
    اماني وديع فرح معهد بحوث الأمصال واللقااحات البيطرية
    نصر محروس حجازي معهد بحوث المصالو اللقااحات البيطرية
Agris Categories Animal diseases
AGROVOC
TERMS		 Babesia equi. Horses. PCR.
Publication Type Journal
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