Trials for production of a combined living attenuated sheep pox and Rift Valley fever vaccine
Abstract: Several formulae containing sheep pox (SP) vaccine (105.5 and 104.5 TCID50/ml) and attenuated Rift Valley fever (RVF) vaccine (10 7.5 , 105.5 TCID50ml ), were prepared and lyophilized together with lactabumin sucrose stabilizer. The different combined formulae were tested for sterility, safety against RVF in mice and lambs; while the safety and efficacy against SP were applied in susceptible lambs. On the other hand, the potency of the prepred vaccine was tested and proved that the types. 1,4,5 and 6 gave acceptable PD50 (< 0.02/ml ) for RVF in adult mice, as well, the potency of the types 1 and 6 representing the highest and the lowest dilutions of RVF vaccine (107.5 and 105.5 TCID50 ml) with the 2 dilutions of SP vaccine (105.5 and 104.5 TCID50ml), was tested in susceptible lambs and recorded a protective difference of log titer (> log102.5). The obtained results stated that the prepared combined SP vaccine and attenuated RVF vaccine proved to be sterile, safe, potent and efficient for vaccination against RVF and SP diseases. Confimatory specific serological test ( serum neutralization test ) was carried out on the sera of vaccinated sheep with combined vaccine, it recorded protective neutralizing indices for SP and RVF (>1.5 and >1.7 respectively) 21 days post vaccination .
Publication year 2004
Availability location معهد بحوث الامصال و اللقاحات البيطرية - القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City cairo
serial title Proc. Thel st Intern. Conf. Vet. Res. Div., NRC, Cairo, Egypt, February 15-17th 2004
Department Rift valley Research
Author(s) from ARC
External authors (outside ARC)
    M.M. Taha
    S.S. Samir
    Iman M.S. Shalakamy
Agris Categories Animal diseases
AGROVOC
TERMS		 Capripoxvirus. Rift valley fever virus. Vaccines.
Publication Type Conference/Workshop
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