Evaluation of using (Lysozyme extract) antigen of virulent mycobacterium Bovis for detection of Bovine tuberculsis
Abstract: Lyozyme extract of Mycobacterium bovis was prepared from cells,which were previously treated with acetone, ethyl alcohol-ether, and chloroform. Cells were dried and suspended in Tris HCI, (pH 7.5)containing Lysozyme and filtered sterilized.
It was found that lysozyme extract and mammalian PPD tuberculin, at equal protein concentratation, elicetd comparable delayed-type hypersensitivity responses in cattle naturally exposed to M.bovis. ELISA results indicated that the values of sensitivity in mammalian bovine PPD and lysozyme extract antigen were (84%, 88% and 92%), while the specificity was (71%,85.7% and 85.8%), respectively. Accuarcy of positive predication for each of three antigens was (91.3%,95.6% and 95.8%) and accuarcy of negative predication (55.6%, 66.7% and 75%). Efficiency of predication was (81.3%, 87.5% and 90.6%) and finally, error of predication was (18.7%, 12.5% and 9.4%) respictively.
These obtained results indicated that lysozyme extract antigen is better than PPD (mammalian and bovine) for immunizing non-specific reactions in diagnosis of bovine tuberculosis by means of skin test or measuring humoral immune response using ELISA.
Publication year 2004
Availability location مكتبة معهد بحوث الامصال و اللقاحات البيطرية- س السكة البيضاء-العباسية-القاهرة
Availability number
Organization Name
City المنوفية
serial title مجلة الطب البيطري بالمنوفية Minufiya. Vet.J
Author(s) from ARC
External authors (outside ARC)
    د.أشرف عواد عبد التواب كلية الطب البيطري بمشتهر
Agris Categories Animal diseases
AGROVOC
TERMS		 Bovinae. Elisa. Lysozyme. Mycobacterium bovis.
Publication Type Journal
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