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Conjugation of clostridium Chauvoei and clostridium septicum hyperimmune sera with Fluorescin isothiocyanate for serological uses
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Abstract: Hyperimmune sera were prepared in sheep against Clostridium chauvoei (C.chauvoei) and Clostridium septicum (C.septicum)where their immunoglobulins were precipitated using saturated ammonium sulfate solution. The concentration of such immunoglobulins was adjusted to be 20mg/ml then conjugated with fluorescin isothiocyanate. The direct fluorescent antibody technique was carried out on culture smears of the 2 strains and muscle sections prepared from infected Guinea pigs by each strain separately. This technique was carried out in a homologous and a heterogeneous manner using the prepared conjugates resulted in barely visible reactions indicating that there was very low or even no cross reaction between Clostridium chauvoei (C.chauvoei)and Clostridium Septicum (C.septicum) confirming the possibility of the use of fluorescent antibody technique to distinguish between them.
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| Publication year |
2006
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| Pages |
313-317
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| Availability location |
معهد بحوث الامصال واللقاحات البيطرية -القاهرة - ش السكة البيضاء - العباسية ص ب 131
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| Availability number |
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| Organization Name |
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| City |
المنوفية
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| serial title |
المؤتمر العلمي الرابع لكلية الطب البيطري جامعة المنوفية
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| Author(s) from ARC |
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| External authors (outside ARC) |
أ.د. عبد السلام زكي أمين
معهد بحوث الأمصال واللقاحات البيطرية
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Immune serum.
Immunological techniques.
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| Proposed Agrovoc |
الكلوستريديم شوفاي;Clostridium septicum;
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| Publication Type |
Conference/Workshop
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