Isolation and identification of serotype O of foot and mouth disease virus from imported bulls and its correlation to the current used vaccine strain O1/3/1993
Abstract: In April 2006, clinical signs of foot and mouth disease (FMD) were observed among bulls imported from Ethiopia at quarantine station in Ismalia governorate. The FMD was notified upon arrival of the bulls. Inoculation of saliva and OP fluids from these bulls in monolayer of primary calf kidney cells resulted in isolation of a virus that was identified as serotype O FMD virus by double sandwich enzyme linked immunosorbent assay (DS-ELISA). This was subsequently confirmed by RT-PCR. ELISA-FMDV-3ABC was carried out to determine the approximately date at which the outbreak started in the country of origin. Six out of 8 sera collected from the same infected bulls had antibodies against FMDV-3ABC polyprotein antigen; probably the infection took place several days before the animals were put on transport. By the two-dimensional serum micro-neutralization test, antigenic relationship between the isolated type O FMD virus and the current used vaccine strain 01/3/93 was sufficiently high to use vaccine prepared from this strain to control this outbreak.
Publication year 2006
Pages 91-100
Availability location مكتبة كلية الطب البيطري جامعة القاهرة - مكتبة معهد بحوث الأمصال واللقاحات البيطرية
Availability number
Organization Name
City القاهرة
serial title المؤتمر الدولي لبحوث الطب البيطري
Author(s) from ARC
External authors (outside ARC)
    سمير محمد على معهد بحوث الأمصال واللقاحات البيطرية
Agris Categories Animal diseases
AGROVOC
TERMS		 Aphthovirus. Bulls. Isolation techniques. Vaccines.
Proposed Agrovoc RT-PCR;1D gene;r-value;
Publication Type Conference/Workshop
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