Detection of FMD virus using a dot immunoblot and RT-PCR from field samples
Abstract: Dot immunoblot assay (DIA) and reverse transcriptase polymerase chain reaction (RT-PCR) were performed to detect foot and mouth disease virus (FMDV) in field samples. FMDV is identified by DIA as a dark blue colored dot using a procedure based on the principle of enzyme linked immunosorbent assay and 4-chloro-1-naphthol as a substrate. All T.E and 5 of nasal swaps tested positive by DIA. RNA extracted from tongue epithelium (T.E) and nasal swap samples were amplified using RT-PCR and two specific primers to ID gene of FMDV serotype O1. The two primers were designed to amplify DNA fragment about 300 bp in length. All T.E and 6 of nasal swaps demonstrated fragments of about 300 bp in length. The five positive nasal swaps with DIA also tested positive by RT-PCR. DIA and RT-PCR were observed by rapid, specific, inexpensive and sensitive techniques for detection of FMD virus.
Publication year 2004
Availability location مكتبة كلية الطب البيطري بمشتهر - مكتبة معهد بحوث الأمصال واللقاحات البيطرية
Availability number
Organization Name
City بنها
serial title المؤتمر العلمي الأول لكلية الطب البيطري بمشتهر
Author(s) from ARC
External authors (outside ARC)
    حليمة محمد الوطني معهد بحوث الأمصال واللقاحات البيطرية
    عبير طلعت معهد بحوث الأمصال واللقاحات البيطرية
Agris Categories Animal diseases
Proposed Agrovoc Dot Immunoblot Assay;RT-PCR;
Publication Type Conference/Workshop
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