RT-PCR amplification of 1D and 3D genes as a tool for rapid diagnosis of foot and mouth disease virus in infected and carrier animals
Abstract: 1D and 3D coding regions of foot and mouth disease virus (FMDV) were determined using reverse transcriptase polymerase chain reaction (RT-PCR). Genomic RNA was extracted from tongue epithelium (T.E) and oesophageal pharyngeal (OP) fluids collected from a bull inoculated with FMDV strain O1/3/93 and nasal swaps collected from apparently healthy 30 cattle and 20 sheep. Two primers for each gene were designed. Gel electrophoresis of the running RT-PCR products revealed a single band about 300 base pair in length for 1D and about 1000 base pair in length for 3D. the determined 1D and 3D genes in T.E and OP samples were detected in 3 out of 50 nasal swaps tested. The significance of detecting 1D and 3D in the extracted RNA as a rapid method for diagnosis and epidemiology of FMD was discussed.
Publication year 2004
Pages 25-34
Availability location مكتبة الجمعية المصرية للعلوم الطبية البيطرية
Availability number
Organization Name
City القاهرة
serial title مجلة الجمعية المصرية للعلوم الطبية البيطرية
Author(s) from ARC
External authors (outside ARC)
    ليلى الشهاوي معهد بحوث الأمصال واللقاحات البيطرية
Agris Categories Animal diseases
AGROVOC
TERMS		 Aphthovirus. Asses. Diagnosis.
Proposed Agrovoc FMDV 1D gene;FMDV 3D gene;
Publication Type Journal
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