Rapid Detection of Equine Herpes virus(EHV-1) from infected Tissues using Polymerase chain reaction (PCR)

Abstract: The polymerase chain reaction (PCR) was applied to detect the local strain of EHV-1 from aborted foetal tissues. Specific forward and reverse primers were selected to amplify a specific region of the glycoprotein H (gH) of EHV-1, these specific primers succeeded to amplify DNA fragment about 636 bp in size from specific pathogen free (SPF). Embryonated chicken eggs infected through the chorioallantoic membrane (CAM) with local isolate of EHV-1, infected baby Hamster kidney cell (BHK-21)with the same virus as well as suspected EHV-1 aborted foetal tissues.
Publication year 2004
Pages 404-412
Availability location معهد بحوث الامصال واللقاحات البيطرية
Availability number
Organization Name
City اسيوط
serial title المؤتمر العلمي الحادي عشر لكلية طب بيطري جامعة اسيوط
Author(s) from ARC
External authors (outside ARC)
    أشرف محمد عباس معهد بحوث الأمصال واللقاحات البيطرية
    مجدي موسى حسنين معهد بحوث الأمصال واللقاحات البيطرية
Agris Categories Pests of animals
Proposed Agrovoc فيروس هيربز الخيول;اختبار تفاعل البلمرة المتسلسل;
Publication Type Conference/Workshop
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