Characterization of immunological differences between Pasteurella multocida isolates of avian origin
Abstract: SDS-PAGE and western immunoblotting were used to investigate the protein antigens of P.multocida local field isolate known to cause fowl cholera epornitics in comparison with reference standard stains. Patterns obtained from protein extract and coomassie blue staining were similar with quantitative differences in the molecular weights of protein present in the range between 33.8-42.6 KDA . The antigenic diversity and immunological variations among avian isolates and standard reference P. multocida strains were investigated by western blot analysis with four groups of standard antisera for Heddleston serotype A, serotypes 1,3,4 and D:11.All blotted antigen components of field or standard reference strain reacted variably with the antisera used reflecting to some to some extent the antigenic diversity of field isolates (except the local field duck isolates) indicating immunological differences among field isolates. These results reflect the importance of using polyvalent local vaccine for protection of avian species against fowl cholera.
Publication year 2000
Pages 655-664
Availability location معهد بحوث الامصال واللقاحات البيطرية
Availability number
Organization Name
serial title SCVNJ,III
Author(s) from ARC
External authors (outside ARC)
    د.صفية طه قنديل معهد بحوث الأمصال واللقاحات البيطرية
    د.ساهر مكين جرجس معهد بحوث الأمصال واللقاحات البيطرية
Agris Categories Animal diseases
AGROVOC
TERMS		 Fowl cholera.
Proposed Agrovoc p.multocida;
Publication Type Journal
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