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Antigenic comparative studies between vaccinal and virulent strains of pigeon pox virus
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Abstract: Local pigeon pox virus (PPV) from naturallyinfected pigeon was isolated and identified. The isolated virus was inoculated on CAM of SPF and it produced specific pock lesion of pox virus.Pathogenicity test was applied by inoculating the isolated virus in susceptible squabs and it produced typical pox lesions. The isolated virus was identified by neutralization test and agar gel precipitation test using specific antisera. Comaprative polypeptide analysis was carried out on SDS-PAGE to identify the difference between the vaccinal strain (Hungarian) and the local field isolate. The polypeptide ranged from 194.2 and 20.0 kDa with total molecular weight (MW) of 1363.598 and 1183.83 respectively for the two strains and the total viral protein of the two strains were estimated by using spectrophtometer and the values were 0.1353 g/dI and 0.1701 g/dI for the vaccinal and the local isolate strain respectively.
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Publication year |
2007
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Pages |
8-13
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Availability location |
معهد بحوث الامصال واللقاحات البيطرية
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Availability number |
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Organization Name |
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City |
الزقازيق
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serial title |
Zagazig veterinary journal
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ISSN |
1110-1458
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Author(s) from ARC |
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External authors (outside ARC) |
د.أميرة عبد النبي السعيد
معهد بحوث الأمصال واللقاحات البيطرية
عبد الرازق بدوي عبد الرازق
معهد بحوث الأمصال واللقاحات البيطرية
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Agris Categories |
Animal diseases
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Proposed Agrovoc |
virulent strain;virulent strain;pox vaccine;
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Publication Type |
Journal
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