Immue response of mice to recobinant vaccina virus expressing the vesicular stomatitis virus new-jersy nucleoprotein
Abstract: A recombinant vaccinia virus that expressing the nucleoprotein gene (Ngene) of vesicular stomatitis virus New-jersy serotype (rVNNJ)was constructed under the control of the P7.5 promotor, using the lac.Z co-expression transfer vector Psc11.recombinant virus was generated by homolgus recombination of the insertion vector with the WR strain of vaccinai virus. The recombinant protein characterized by western blot and radioimmunoprecipitation using anti-VSV serum was shown to have a molecular weigh of 47 KDa. Mice vaccinated with rVNNJ did not develop neutralizing antibodies before challenge with VSV, while Anti-N antibody was detected using bacterially expressed NNJ protein as a coating antigen for ELISA plates Seventy percent of mice vaccinated intraperitioneal and intranasal and boosted with rVNNJ, 80% of mice vaccinated intraperitoneal with both rVNNJ and rbNVNJ and bbosted with rbNVNJ, 20% of mice vaccinated intradermal with rVNNJ boosted with the same route, in which 10% of mice vaccinated intraperitoneal with WR strain of vaccinia virus were survived the challenge with VSV. Vaccination trials in mice indicated that the nucleoprotein is cabable of elicting a protective immune response and that the N protein should be considered in recombinant vaccines
Publication year 1997
Availability location معهد بحوث الامصال واللقاحات البيطرية-ش السكة البيضاء-العباسية-القاهرة
Availability number
Organization Name
City اسيوط
serial title المؤتمر العلمي الرابع جامعة اسيوط
Author(s) from ARC
External authors (outside ARC)
    د/شابير احمد معمل البيولوجيا - جامعة كليفورنيا - ديفيس
    د/لزلي جونز
    د/تيلوهن يلما
Agris Categories Animal diseases
AGROVOC
TERMS		 Nucleoproteins.
Proposed Agrovoc Mice--Diseases;
Publication Type Conference/Workshop
↑