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دراسات على بعض الاختبارات السريعة والحساسة للتعرف على فيروس الإجهاض المعدي في الخيول
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Studies on some RAPID and sensitive techniques for detection of Equine Herpes virus
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Abstract: Specific pathogen free (SPF) Emberyonated Chicken Egg (ECE), 11-13 days old were inoculated by locally isolate of EHV- 1 for propagation and adaptation and antigen preparation. Dot – immunobinding assay, imnunofluoroscence and western blot were applied in this study for rapid detection of EHV-1 dot – immunobinding assay proved its sensitivity and reliability for rapid detection and titration of EHV-1 antigen. While IF could detect EHV-1 as early as 8 hours post inoculation of CAMs, the reaction increased gradually till it reached is maximal at 18 hours post inoculation. Also, western blot proved its ability for detection the most immunogenic epitops of EHV- 1,29,5 K Da
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| Publication year |
2004
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| Pages |
463-470
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| Availability location |
معهد بحوث الامصال و اللقاحات البيطرية - القاهرة - ش السكة البيضاء - العباسية
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| Availability number |
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| Organization Name |
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| City |
قناة السويس
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| serial title |
مجلة الطب البيطري لقناة السويس
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| ISSN |
1110-6298
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Equine herpesvirus.
Herpesviridae.
Rapid rural appraisal.
Viruses.
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| Proposed Agrovoc |
Westren blot;immunofluroscent technique;
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| Publication Type |
Journal
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