Preparation of inactivated freeze - dried RVF vaccine

Abstract: Attentions were directed to develop a lyophilized RVF vaccine to overcome the disadvantages associated with the poor keeping quality, as well as the short immunizing antibody titre of the conventionally produced RVF liquid – inactivated vaccine.
The used virus seed having an initial titre of 107 TCID50 was inactivated by 0.1M binary ethyleneimine (BEI) at a concentration of 1% full inactivation of the virus was achieved after incubating the mixture at 37o C for 6 hours.
Tetanus toxoid was added at 3 different concentration, then an equal volume of stabilizer was also added to the inactivated virus. The vaccine was dispensed into 2ml vials and freeze – dried.
The safety and potency in mice showed that RVF vaccine combined to tetanus toxoid 200 limit flocculation (L. F) concentration is absolutely safe and potent
So , this innovated lyophilized RVF vaccine combined to tetanus toxoid will solve the disadvantages of the already produced liquid RVF vaccine besides the prophylactic measures of tetanus.
Publication year 2003
Pages 1239-1247
Availability location معهد بحوث الامصال و اللقاحات البيطرية - القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City الجيزة
serial title المجلة المصرية الزراعية للأبحاث- مركز البحوث الزراعية
Author(s) from ARC
External authors (outside ARC)
    د.ايمان الشلقامي معهد بحوث الأمصال واللقاحات البيطرية
    د.سامي سعد محمد
Agris Categories Animal diseases
AGROVOC
TERMS		 Dried products. Rift valley fever virus.
Publication Type Journal
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