Adaptation and propagation of the Egyptian strain of pigeon pox on different types of cell cultures.
Abstract: A virulent Egyptian strain of pigeon pox virus (PPV), isolated from a local outbreak, was adapted and attenuated in tissue cultures. The virus was primarly purified and propagated in 9-11 day-old embryonated chicken eggs for three successive passages. The virus was adapted on chicken embryo fibroblast (CEF) cells, clear cytopathic effect (CPE) appeared by the 7th passages. This virus was transferred for adaptation and attenuation in African green monkey kidney cells (VERO) , clear CPE appeared by the 17th passage. The passage was continued with recording the titre (in tissue culture and embryonated chicken eggs) and the pathogenicity (in susceptible squabs) every 5 passages up to 27 passages. The results revealed that the adapted PPV reached its maximum titre (105.4 TCID 50/ml at the 27th passages on VERO cells and 107.2 EID 50/ml in embryonated chicken eggs for the same passage) and lost its pathogenicity after 27 serial passages in tissue cultures was monitored from the 1st up to 120th hour post inoculation, beside the detection of intracytoplasmic inclusion bodies specific to pox viruses.
Publication year 2005
Pages 4
Availability location معهد بحوث الامصال و اللقاحات البيطرية - القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City القاهرة
serial title مجلة الجمعية الطبية البيطرية المصرية
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Biological differences. Cell culture. Pigeons.
Proposed Agrovoc PIGEON POX;Egyptian strain;
Publication Type Journal
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