Trials for production of inactivated pigeon pox vaccine from the local isolate
Abstract: In the present study, inactivated pigeon pox virus (PPV) vaccines were prepared from a virulent local isolate. The virus was inactivated by treatment with formalin and binary ethyleneimine (BEI) at different ratios and incubation intervals. Inactivation of PPV with formalin was applied at concentrations 0.5%, 1%, 2 % incubated for 24 hours with residual neutralization every 2 hours intervals. It was found that 0.5 % concentration for 24 hours incubation was the best. The inactivation process of PPV with BEI was applied at 1%, 2%, 3% concentration incubated for 24 hours, with residual neutralization every hour intervals. The virus was completely inactivated after 18 hours incubation by 1 % BEI.
The inactivated viruses were absorbed on aluminum hydroxide gel 1:1 and incubated for 6 hours. The BEI inactivated PPV vaccine was better than formalin inactivated one due to the full inactivation efficacy of BEI.
The antibody levels were estimated by serum neutralization test, where specific antibodies appeared from the 1st week post vaccination and reached its peak on the 3rd week and remained protectable until the 6th month post vaccination. The prepared vaccine proved to be sterile, safe and potent.
Publication year 2005
Organization Name
City El- Mansoura
serial title 4th International scientific conference at Mansoura
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Experimentation. Immune serum. Pigeons. Vaccines. Viroses.
Publication Type Conference/Workshop
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