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Trials for preparation and evaluation of peroxidase and fluorescent isothiocyanate conjugated antisera against equine herpesvirus-1.
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Abstract: A preliminary study was conducted to prepare a polyclonal antibody against local strain of equine herpsvirus-1 (EHV-1), labeled with horseradish peroxidase and fluorescein isothiocynate. The prepared antiserum in rabbit was conjugated by the most cheaper and easier method (periodate method). It was divided into two parts ; the first one was conjugated with peroxidase which gave strong positive reaction at dilution of 1/2000. The second part was conjugated fluorescin isothiocyanate which give the strongest positive reaction at dilution of 1/4000. Both conjugates were succeeded to show its efficiency and liability for detecting EHV-1 when tested against field- samples within its test proper.
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| Publication year |
2006
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| Pages |
7
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| Organization Name |
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| City |
Minufiya
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| serial title |
Minufiya Vet. Journal
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Equine herpesvirus.
Immune serum.
Immunofluorescence.
Peroxidases.
Rabbits.
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| Publication Type |
Journal
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