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Preparation of peroxidase conjugated and non conjugated antisheep IgM immunoglobulin
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Abstract: Peroxidase conjugated and non-conjugated antisheep immuno-globulin-M (IgM)were prepared from rabbit hyperimmune serum. The optimal dilutions of both products were determined by checkboard ELISA method which proved their efficacy. Their titres were 1:2000 and 1:100, respectively . Both peroxidase conjugated and non-conjugated antisheep IgM, in comparing with imported standardized product were applied on 28 serum samples collected from sheep infected with Rift Valley Fever virus (RVFV) to detect IgM antibodies against RVFV using both indirect ELISA and IgM capture ELISA. The results revealed that non-conjugated antisheep Igm using Igm capture ELISA was superior to peroxidase conjugated antisheep IgM using indirect ELISA for detection of IgM antibodies.
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| Publication year |
2007
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| Pages |
2
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| Organization Name |
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| City |
القاهرة
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| serial title |
مجلة البحوث الزراعية
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| Author(s) from ARC |
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| External authors (outside ARC) |
د.تراضي عبد الفتاح سيد
معهد بحوث الأمصال واللقاحات البيطرية
كريم الدين زكي حسن
المعمل المركزي للرقابة على المستحضرات الحيوية البيطرية
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AGROVOC
TERMS |
Antibodies.
Immune serum.
Immunoglobulins.
Peroxidases.
Sheep.
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| Publication Type |
Journal
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