Efficacy of Solubilized Protein Antigen Extracted from Mycobacteria For Detection of Bovine Tuberculosis
Abstract: Extraction of new native antigen fraction from Mycobacterium bovis without massive degradation of proteins by Triton X-100 was prepared. To characterized the nature of there composition, the triton X-100 solubilized protein (TSP) antigens was fractionated by Triton X-114 phase partitioning, to the aqueous phase (TSP aqueous phase) (TSP- Aq) and detergent phase (TSP detergent phase) (TSP-Dt). Original TSP, TSP-Aq were investigated and compared with the purified protein derivative (PPD) by measuring the cell mediated immunity using the lymphocyte blastogenesis assay (LBA) and skin sensitivity test, as well as measuring the serum antibodies levels using ELISA in experimentally infected Guinea pigs with either M. bovis or M. avium, in addition to naturally infected cattle. The lymphoproliferative responses to the TSP aqueous fraction in infected Guinea pigs with M.bovis were significantly higher than those to the PPD and unfractionated TSP. In contrast, the antibody response to TSP – Aq can be used as a T- cell antigen associated with protective immunity against bovine tuberculosis
Publication year 2005
Availability location معهد بحوث الامصال واللقاحات البيطرية- ش السكة البيضاء - العباسية ص ب 131
Availability number
Organization Name
City اسيوط
serial title المؤتمر العلمي الثامن للجمعية المصرية لأمراض الماشية بالإشتراك مع كلية الطب البيطري - جامعة اسيوط
Author(s) from ARC
External authors (outside ARC)
    عصام امين نصر معهد بحوث الأمصال واللقاحات البيطرية
Agris Categories Animal diseases
Proposed Agrovoc M.bovis;Purified protein derivative;Cell meditaed immunity;
Publication Type Conference/Workshop
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