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Detection of ALPHA toxin gene of Clostridium perfringens Type-A isolated from Diseased Rabbits and Chicken by polymerase chain reaction
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Abstract: C.perfringens strains isolated from chicken (necrotic enteritis) and rabbits (enterotaxaemia) were typed using mice serum neutralization test (SNT) as biological assay that all toxigenic C.perfringens isolates were belonged to serotype A and it was the dominant type, and the alpha toxin is considered to be a key virulence determinant type , and the alpha toxin is considerd to be a key virulence determinant in necrotic enteritis in chickens and enterotoxaemia in rabbits.Comparison of the PCR proved to be effectious, accurate, sensitive, specific, faster and sueprior test than SNT. The results of PCR techbique indicated that the amplified DNA products of C.perfringens isolates from rabbits and chicken were in the same molecular size.
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| Publication year |
2007
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| Pages |
175-187
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| Availability location |
معهد بحوث الأمصال واللقاحات البيطرية-القاهرة - ش السكة البيضاء - العباسية ص ب 131
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| Availability number |
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| Organization Name |
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| City |
بنها
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| serial title |
Benha veterinary medicine journal
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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| Publication Type |
Journal
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