Conjugation of Lipopolysacharide brucella Antigen with Fluorescein isothiocyanate for detection of brucellosis
Abstract: Serological tests were developed for the detection of antibodies against brucella species. In the present study. Lipopolysacharide of Brucella abortus S99 was conjugated with fluorescein isothiocyanate (FITC) and for detection of brucellosis in comparison with other serological tests as Rose bengal test (RBT), competitive enzyme linked immunosorbent assay (I-ELISA). The sensitivity of fluorescent antibody technique (FAT) and c-ELISA was 100 % and 90 % respectively, while that of I-ELISA and RBT was 75 % and 81% respectively in cattle sera. The relative specifity of FAT was 100%, 96% and 92.8 % in cattle, sheep and goat sera, respectively. The FITC is the test of choice for diagnosis of Brucellosis because it has the best sensitivity and specificity values comparable to the C-ELISA, I-ELISA and RBT.
Publication year 2009
Pages 73-78
Availability location ش السكة البيضاء - العباسية-القاهرة
Availability number
Organization Name
City zagazig
serial title zagazig veterinary medical journal
ISSN 1110-1458
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Antigens. Brucellosis.
Publication Type Journal
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