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The use of VP60 RT-PCR to overcome limitations with the current diagnostic approaches to rabbit haemorrhagic disease virus.
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Abstract: Rabbit viral hemorrhagic disease (RVHD) is a highly contagious. Highly fatal, per acute and acute viral disease of both wild and domestic rabbits caused by rabbit haemorrhagic disease virus (RHDV). Dependence on single test for the diagnosis of RHDV is often misleading. We investigated several RHDV outbreaks in the Egyptian environment to compare results obtained by the current diagnostic approaches. The results of heamagglutination (HAT) revealed that 22 out 61 suspected RVHD samples (36.06%) were positive using slide HAT. Using plate HAT. At 4oC, only 40 samples were positive despite the presences of characteristic clinical and pathological signs. RHDV was isolated from 25 different outbreak samples. RVHD diagnosis was confirmed by SDS-PAGE, western blotting and reverse transcriptase/ polymerase chain reaction (RT/PCR). Minor differences between the different isolates were found using SDS-PAGE and western blotting analysis. Different isolates of positive and negative HA activity gave RT –PCR products for three VP60 genomic regions tested. Only 2 out of 4 tested samples using IEM were positive. The histopathological picture provided important diagnostic information. Our data demonstrate the extenstive presence of non-haemagglutinating RHDV in the Egyptian environment and the inevitability of the shift towards use of molecular diagnostic techniques based on RT-PCR.
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| Publication year |
2008
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| Pages |
217-237
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| Availability location |
ش السكة البيضاء - العباسية -القاهرة
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| Availability number |
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| Organization Name |
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| City |
cairo
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| serial title |
Egyptian ournal of virology
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| ISSN |
1685-1687
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| Author(s) from ARC |
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| External authors (outside ARC) |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
PCR.
Rabbits.
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| Publication Type |
Journal
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