Methods for preparation H7 flagellin and polyclonal antibodies for detection of H7 antigens of E/coli O157:H7
Abstract: E coli of the serotype O157: H7 has recently been isolated from cattle and human fecal samples in Egypt. The aim of this study is to develop an effective method for extraction of flagella from E. coli O157: H7.

Flagella were prepared through the following steps: dissociated by incubation of bacteria, ethanol treatment, supernatant collection and finally precipitated by centrifugation. Flagellar filaments in E. coli O157: H7 were demonstrated by transmission electron microscopy and by PCR. H7 flagellin proteins were shown to be immunogenic. They induced a systemic immune response against those H7 proteins when injected S/C into animals or when given orally.

Polyclonal antiserum raised to H7 flagellin proteins reacted with intact E. coli O157: H7. also, this antiserum reacted strongly with E. coli O11: K+H34 and O156: K-: H23. in effect, the prepared polyclonal sera have the potential to serve as high- quality diagnostic reagent.
Publication year 2008
Pages 119-126
Availability location ش السكة البيضاء - العباسية -القاهرة
Availability number
Organization Name
City القاهرة
serial title مؤتمر الشرق الأوسط وشمال افريقيا لبحوث الثروة الحيوانية
Author(s) from ARC
External authors (outside ARC)
    محمود عبد الواحد رافع المعمل المركزي للنظم الزراعية الخبيرة
Agris Categories Animal diseases
AGROVOC
TERMS		 Antigens. Polyclonal antibodies.
Publication Type Conference/Workshop
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