Studies on rinderpest virus in theileria annulata infected lymphoblastoid cells
Abstract: A vaccine strain of rinderpest virus (Kabete O) was passaged for three successive times in Theileria annulata infected lymphoblastoid cells. The virus showed its characteristic cytopathic effects in T. annulata infected cells without any effect cells on the schizont. Specific cell aggregations, intracytoplasmic inclusions and cyncytia formation appeared within 48-72 hours post inoculation. Immunofluorescent specific rinderpest intracytoplasmic antigen within 24-48 hours post inoculation. Giemsa staining showed healthy schizonts before infection with the rinderpest virus vaccine and its prescence as intact schizontal bodies in the cells during the viral infection of their host cells. The virus titre reached 107 TCID50/ml by the third passage with reduction in the time of harvestation from 7 days (for the 1 st passage) to 3 days (for the 3rd passage). This work planned as a preliminary study to produce a combined tissue culture vaccine against rinderpest and T. annualata.
Publication year 1994
Pages 104-110
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City الزقازيق
serial title zagazig veterinary journal
ISSN 1110-1458
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Cells. Rinderpest. Theileria annulata.
Publication Type Journal
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