Detection of PPR virus in vero cells using fluorescent antibody technique
Abstract: The growth curve of pest des petits ruminants virus (PPRV) On VERO cells indicated that the highest titre of cell associated virus was 5.6 log10 TCID50/ml obtained 48 hours post inoculation, while the highest titre of the cell free virus was 5.7 log10 TCID 50/ml, 72 hours post inoculation. The total virus yield had a titre of 504 log10 TCID50/ml after 72 hours of infection.
Fluorescent antibody technique (FAT) showed a diffuse specific fluorescence emiting from the cytoplasm of infected VERO cells with pest des petits ruminants virus (PPRV), 12 hours post infection. A granular intracytoplasmic intermittent fluorescence was developed 24 hours post infection. At 48 hours post infection, the infected cells showed granular and diffuse fluorescence shining intracytoplasmic. As the cytopathic effect (CPE) of PPRV was progressed on the 72 hours post infection, the cells showed clearly CPE in the form of intermingled anastomosed cells with faint greenish fluorescence. On the 96 the hours post infection. PPRV infected cells showed themselves with heavily developed CPE and intense intracytoplasmic granular and diffused specific fluorescence. Some cells showed intranuclear fluorescing granules.
This study was planed to detect the optimal time of havestation to obtain a highest titre of PPR virus on VERO cells as a first step to prepare a highest titre of PPR virus on VERO cells as a first step to prepare an attenuated tissue culture PPR vaccine and to study the possibility of early diagnosis of PPR using fluorescent antibody technique.
Publication year 1994
Pages 1994-119
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City الزقازيق
serial title zagazig veterinary jornal
ISSN 1110-1459
Author(s) from ARC
Agris Categories Animal diseases
AGROVOC
TERMS		 Cells. Immunofluorescence.
Publication Type Journal
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