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Genetic identification of the foot and mouth disease virus caused 2006 outbreak in Egypt
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Abstract: In early February 2006, afoot and mouth disease (FMD) outbreak has struck cattle and buffaloes in different localities of Egypt exertinf severe economic losses to livestock industry. Representative specimens(tongue epithelium and foot vesicular fluid) were collected from severly infected foreign (imported from Ethiopia) and local cattle in different governorates (Ismailia, Sharqia and Behairah). Severla assays of reverse transcription (RT) using random decamer promers, followed by FMDV VP-1 based polymerase chain reaction (PCR), were used for rapid identification of the causative agent as a FMDV in all clinical specimens. FMDV specific promers were then utilized to determine the outbreak FMDV serotype. The specific PCR amplification products 9ampplicons) were purified and subjected to direct nucleotide sequencing. Blast searches, multiple alignments and phylogenetic analysis of the nucleotide sequence data revealed that outbreak FMDV is a serotype"A" which is a new serotype incursion to Egypt. Direct sequencing of teh PCR amplicons was proved a revelant discriminative tool for genetic characterization of FMDV strains/isolates. Results of this endeavor initiated the potential to produce a bivalent FMDV c=vaccine, containing both of serotypes A and O1, for teh first time in Egypt.
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| Publication year |
2007
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| Pages |
193-206
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| Availability location |
القاهرة - ش السكة البيضاء - العباسية
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| Availability number |
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| Organization Name |
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| City |
الجيزة
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| serial title |
المجلة العربية للتكنولوجيا الحيوية ( مجلة دولية للعلوم البيولوجية التطبيقية)
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| ISSN |
1110-6875
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Foot and mouth disease.
Genetics.
Viruses.
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| Publication Type |
Journal
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