Application of PCR as a diagnostic tool of clinical mastitis in dairy cattle in comparison to traditional methods
Abstract: Commercial dairy farms had serious mastitis problems in spite of its control hygienic measures, for detection of this problem, a total of 3000 and 1500 lactating cows& buffaloes, respectively, were examined for clinical mastitis in 8 different farms at Ismailia Governorate. 130 milk samples were taken from 108 dairy animals suffering from clinical mastitis. The Bacteriological examination revealed that 56(43.1%), 34(26.2%), 8(6.1%), 4(3.1%) and 4 (3.1%) from clinically mastitic milk samples were positive for Staphylococcus epidermidis, staphylococcus aureus, Streptococcus uberis. Bacillus anthracoid and Enterobacter agglomerans, respectively. While 24(18.5%) of the samples were negative. Polymerase chain reaction (PCR) was also conducted to Staphylococcus aureus isolates as amajor pathogenic bacteria in cases of mastitis, Genomic DNAs extracted from all S. aureus isolates (n=34), were successfully identified by this PCR assay. PCR aws proved to be specific and sensitive in diagnosis of clinical mastitis.
Publication year 2008
Pages 61-68
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City قناة السويس
serial title المؤتمر العلمي الرابع لكلية الطب البيطري- جامعة قناة السويس
ISSN 1110-6298
Author(s) from ARC
External authors (outside ARC)
    محمد عناني قسم البكتريا والمناعة والفطريات - جامعة قناة السويس
    حمزة عيد قسم البكتريا والمناعة والفطريات - جامعة قناة السويس
    نجلاء حمدي عبد العظيم قسم البكتريا والمناعة والفطريات - جامعة قناة السويس
Agris Categories Animal diseases
AGROVOC
TERMS		 Dairy cattle. PCR.
Publication Type Conference/Workshop
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