Preliminary preparation and evaluation of a local Lysate vaccine for protection of chicken against E. coli infection
Abstract: Four serotypes of E. coli (O1, O2, O6 and O128) were used in preparation of a local polylysate oil vaccine that was evaluated in comparison with a licensed imported one for protection of chicken against E. coli infection. Five groups. Each of 20 chickens were vaccinated with the local vaccine subcutaneously (S/C) and intramuscularly (I/M) using single or double vaccinal dose and boos tered after 2 weeks. Vaccinated and control chickens were challenged 2 weeks post second vaccination with the four virulent E. coli strains. No deaths occurred in all vaccinated chickens except one death case in one group. Survivors were necropsied one week post challenge. No gross lesions were observed in all vaccinated groups compared to those of non –vaccinated one. Polyly-sate vaccine gave similar results to that of the imported vaccine Anti- E. coli antibodies were measured by an indirect enzyme linked immuno-sorbent assay (ELISA) in sera of all vaccinated and challenged chickens utilizing the for E. coli vaccinal antigens as individuals and as a polylysate. Moreover, no genetic variation could be detected among the four serotypes after digestion of their chromosomal DNA with xbai enzyme. In candidates for vaccine preparation to protect chickens from colibacillosis.
Publication year 2007
Pages 365-375
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City Giza
serial title Vterinary medical journal
Author(s) from ARC
External authors (outside ARC)
    امل مصطفى السواح المعمل المركزي للرقابة على المستحضرات البيطرية
Agris Categories Animal genetics and breeding
AGROVOC
TERMS		 Chickens.
Proposed Agrovoc Lysate vaccine;E.coli;
Publication Type Journal
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