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Baculovirus expression of P14 and GP48 of the NADL strain of Bovine viral diarrhea virus and utility for serodiagnosis
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Abstract: A recombinant baculovirus constrcut was generated that expressed p14 and gp48 proteins of the NDAL strain of bovine viral diarrhea virus(BVDVr1448) in insect cells (Sf9). The recombinant protein was antigenically authentic as shown by teh western immunoblot, immunofluorescence, and immunoperoxidase staining of infected Sf9 cells. Crude lysate containing teh recombinant protein(r1448) was used as a coating antigen in an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibody against BVDV. The ELISA was compared to standard virus neutralization test (VNT) for detection of antibody against BVDV in cattle sera. Antibody titers determined by VNT (r=0.9). The recombinant protein was an effective and inexpensive ELISA antigen for detection of antibody against both genotypes of BVDV in reference hyperimmune cattle sera. These findings indicate that the p14 and gp48 proteins of pestviruses are useful for diagnostic tests.
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| Publication year |
1999
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| Pages |
491-511
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| Availability location |
القاهرة - ش السكة البيضاء - العباسية
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| Availability number |
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| Organization Name |
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| City |
Beni-Suef
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| serial title |
Beni-suef veterinary medical journal
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| Author(s) from ARC |
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| External authors (outside ARC) |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
Baculoviridae.
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| Proposed Agrovoc |
viral diarrhea virus;
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| Publication Type |
Journal
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