Evaluation of a recombinant Elisa antigen for Serodiagnosis of bovine viral diarrhea in immune and infected cattle sera in Egypt
Abstract: The recombinant protein (r 1448) in crude lysate of insect cells infected with a previously developed recombinant baculovirus expressing two structural proteins, the uncleocapsid protein (p 14) and the envelope glycoprotein (gp 48) of the NADL strain of bovine viral diarrhea virus (BVDV), was evaluated as a coating antigen in a standard enzyme –linked immunosorbent assay (ELISA) for detection of antibody against homologous and heterologous strains of BVDV. The ELISA was compared to standard virus neutralization test (VNT) for detection of antibody against the local Iman strain of BVDV in sera of calves immunized with the locally produced pneumo-3 inactivated vaccine experimentally infected calves and contact controls. Antibody titers estimated by ELISA were closely correlated with antibody titers estimated by VNT (average r = 0.917). nevertheless, the recombinant protein (r 1448) was effective and inexpensive ELISA antigen for detection of antibody against homologous and heterologous strains of both genotypes of BVDV (BVDV1 and BVDV2) in reference hyperimmune cattle sera. In conclusion, the recombinant antigen (r 1448) is reliable candidate for diagnostic tests of BVDV infections in Egypt.
Publication year 2000
Pages 227-238
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City Giza
serial title veterinary medical journal - faculty of veterinary medicine cairo university
ISSN 1110-1423
Author(s) from ARC
Agris Categories Animal diseases
Proposed Agrovoc Bovine viral diarrhea;
Publication Type Journal
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