Cloning and expression of the nucleoprotein of peste des petits ruminants virus in baculovirus for use in serological diagnosis
Abstract: Paste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis enteritis and pneumonia. The virus is a member of the family paramyxoviridae and the genus morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing countries. We have cloned and sequenced the CDNA of the uncleocapsid (N) gene of the Nigeria 75/1 strain of PPR virus (PPRV). A comparison of its nucleotide and deduced amino acid sequence with those of the N gene of the tissue culture- attenuated strain of PPRV was culture- attenuated strain of PPRV was performed. A divergence of 8.9 and 5.0% was found at the nucleotide and amino acid level, respectively. A recombinant baculovirus that expresses the N protein in insect cells and larvae (Spoodoptera frugiperda) was generated. The recombinant protein, characterized by western blot analysis, was shown to have a molecular weight of 58KDa and was recognized by anti- PPRV antibodies the recombinant protein was used successfully as a coating antigen in an enzyme-linked immunosorbent assay for the serological diagnosis of PPRV.
Publication year 1995
Pages 776-778
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
serial title VIROLOGY JOURNAL
Author(s) from ARC
External authors (outside ARC)
    MILES K.YAMANAKA
    JEREMIAH T. SALIKI
    CHARLES MEBUS,I
    TILAHUN YILMA
Agris Categories Animal diseases
AGROVOC
TERMS		 Baculoviridae. Nucleoproteins. Ruminants. Viruses.
Publication Type Journal
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