Use of PCR and In Situ DNA hyperidization for detection of Aeromonas Hydrophila infection im Nile Tilapia (Oreochromis niloticus)
Abstract: In the present study, plolymerase chain reaction(PCR) was used to detect Aeromonas hydrophila (A.hydrophila) utilizing a primer set specific for AEROLYSIN GENE. A similar PCR was performed to prepare a non-radioactive Aerolysin-specific DNA probe using a PCR Dig-probe kit (Boaehringer-Mannheim). The dig-labeled probe was employed in an in situ hybridization assay for detection of A.hydrophila infection in tissue of experimentally infected Tilapia, Oreochromis niloticu, steps for experimental infection, tissue fixation, prehybridization, hybridization and detection are described. The in situ hyperidization assay developed in this study is proved reliable for safe, rapid and accurate detection of A.hydrophila early infection in fish. Moreover it initiates a potential to trace Kinetics of A.hydrophila infection in fish tissue during different stages of disease.
Publication year 2001
Pages 177-189
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City Cairo
serial title 25th Arab veterinary medical congress
Author(s) from ARC
External authors (outside ARC)
    ايهاب الدين السيد قسم امراض ورعاية السمك-كلية الطب البيطري-جامعة القاهرة
    محمود حشاد قسم الميكروبيولوجي - كلية الطب البيطري-جامعة القاهرة
Agris Categories Animal diseases
AGROVOC
TERMS		 Aeromonas. DNA. PCR. Tilapia.
Publication Type Conference/Workshop
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