Molecular evaluation of some antigenic extracts of Corynebacterium pseudotuberculsis and utility in serodiagnosis
Abstract: Virulence factors of C.pseudotuberculosis, represented by concentrated exotoxin containing culutre filtrate and whole cell lysate (WCL) FROM 3 local isolates of ovine, caprine (serotype I)and buffalo (serotype II) origins, were comparatively analyzed for their antigenic structure and cross-reactive determinants, using sodium dodecyle sulphate-polyacrylamide gel electrophoresis ( SDS-PAGE) and western imunoblotting. Morever, their utility as candidate antigens for serodiagnosis of C.pseudotuberculosis infection in Egyptian sheep and buggaloes was investigated, by an indirect enzyme-linked immunosorbent assay (ELISA) and agar gel immuno-diffusion test (AGIDT). SDS-PAGE analysis of the exotoxins revealed high similarity among the 3 local isolates as determined at protein subunits with molecular masses(Mr) of about 68, 40-41, 39, 31.5, 29, 27 and 20 Kilodaltons (kDa). While, 4 antigens with approximate Mr of 68, 40, 3.15-31 and 29 kDa immunoreacted (ovine) and cross reacted (caprinsand buffalo) in immunoblotting. The exotoxin of ovine isolate showed othr 2 immunoreactive antigens at Mr. of about 20 and 14 kkda. Concerning the WCL, no markded differences could be observed among the 3 local isolates. as numerous immunoreactive/ cross-reactive antigens were estimated at aproximate Mr of 120, 100, 90, 72-68, 64, 40, 31, 29 and 20 kDa. Both WCL and exotoxin antigenic extracts from the local ovine isolate proved effective potential candidates in ELISA and AGIDT for serodiagnosis of clinical and inapparent infections. Agidt, for detection of seropositve sheep and buffalo.
Publication year 2004
Pages 369-385
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City Sharm El-Sheikh
serial title Seventh scientific veterinary medical conference
Author(s) from ARC
External authors (outside ARC)
    أمل مصطفى السواح المعمل المركزي للرقابة على المستحضرات الحيوية البيطرية
    الهام عطا الإبياري
Agris Categories Pests of animals
AGROVOC
TERMS		 Corynebacterium. Evaluation. Extracts. Molecular biology.
Publication Type Conference/Workshop
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