Molecular characterization of mannheimia Haemolytica extracts and utility in Serodiagnosis of pasteurellosis in Egypt
Abstract: Virulence factors of M. haemolytica (A2) and P. trehalosi (T4) local isolates of caprine origin represented by outer membrane proteins (OMP), lipopolysaccharides (LPS) leukotoxin (LKT) and bacterial cell lysate (BCL) as well as OMP of P.multocida (B6) standard strain were comparatively analysed for their antigenic structure and cross-reactive components, using sodium dodecyl sulphate- polyacrylamide gel electrophoesis (SDS- PAGE) and western immunoblotting. Main differences among M. haemolytica and Pasteurella species (p.trehalosi and P. multocida) were observed in their OMP extracts. A 37 kilo Daltons (KDa) antigenic determinant was a M.haemolytica (A2) Specific immunoreative protein subunit. The most unique cross- reacting antigens in OMPs of P. trehalosi (T4) and P. multocida (B6) versus polyclonal anti- M. haemolytica (A2) antiserum were at 66, 44, 43 and 41 KDa that could be potential pasteurella- related surface antigens. The major immunodetected antigenic determinants in all M. haemolytica (A2) extracts analysed were at 100-105, 66-68.41-44,28-29 and 16-18 KDa. Neither SDS- PAGE nor Western immunoblotting analyses revealed marked differences in LPS core LKt and BCL electrophoretic and immu-no-reaction patterns between M. haemolytica and P. trehalosi local isolates in the indirect enzyme- linked immunosorbent assay (ELISA), indirect haemagglutination (IHA) and rapid plate agglutination (RPA) tests, all antigenic extracts of M. haemolytica (A2) were proved effective potential candidates for serovar- wide detection of anti- M. haemolytica antibody in livestock sera (cattle , buffalo, sheep and camel). However, BCL antigen scored higher values then did the OMP then LKt and LPS. The present work extend the epidemiological analyses related to pasteurellosis in Egypt and initiates a potential for development of rapid diagnostic reagents for livestock animals at risk.
Publication year 2004
Pages 321-343
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City القاهرة
serial title مجلة الجمعية الطبية البيطرية المصرية
Author(s) from ARC
External authors (outside ARC)
    عبد السلام زكي
Agris Categories Agricultural research
AGROVOC
TERMS		 Extracts. Molecular biology. Pasteurellosis.
Publication Type Journal
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