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Comparative polymerase chain reaction assays for detection and genotyping of bovine viral diarrhoea viruses in Egypt
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Abstract: Comparative assays of single-step reverse transcription polymerase chain reaction (RT-PCR), utilizing the5' unranslated region (5'UTR) and the non-structural p125 encoding gene of bovine viral diarrhoea virus (BVDV) genome as targets and indirect immunofluorescene (FA) using anti-BVDV E2 monoclonal antibody(antiE2 MAb) were conducted for detection and genotyping of lcal BVDBs. For BVDV isolation, 112 suspected bovine clinical specimens in the form of nasal swabs, buffy coats and sera were obtained from different localities in Egypt(Dakahlia, Kafr El-Sheikh and Domyat). A total of 101 clinical specimens were found free of BVDV by all assays used in this study. FA technique following viral isolation procedure in cell culture identified 8/11 (72.7%) of the viral isolates. The single-step RT-PCR using primers derived from 5' UTR and p125 encoding gene of BVDV genome detected 11/11 (100%) and 10/11 (91%) of the viral isolates, respectively. A nested PCR using another internal primers from teh 5' UTR led to rapid genotyping of BVDVs. The local vaccinal BVDV, the Iman strain was identifid as agenotype2 cytopathic BVDV. aLSO, 9/11 (81.8%) OF The viral isolates were characterized as genotype 2 BVDVs. Findings of this study emphasized the utility of specific primer-derived PCR specially that is based on the 5' UTR, as a sensitive and rapid assay for epidemiological studies and control programs of BVDV infections. Accordingly, it is recommended for routine screening of not only animals but also veterinary biologicals (locallly produced and important) for BVDV contamination
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| Publication year |
1999
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| Pages |
165-170
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| Availability location |
القاهرة - ش السكة البيضاء - العباسية
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| Availability number |
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| Organization Name |
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| City |
اسيوط
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| serial title |
المؤتمر العلمي الخامس - جامعة اسيوط
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| Author(s) from ARC |
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| Agris Categories |
Animal diseases
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AGROVOC
TERMS |
PCR.
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| Proposed Agrovoc |
DIRRHOEA;
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| Publication Type |
Conference/Workshop
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