Molecular and Immunological detection of bovine Herpesvirus-1 in clinical specimens.
Abstract: of bovine herpesvirus-1 (BHV-1) as a target, virus isolation (VI), indirect immunofluorescence (IFA) and In situ immuno-peroxidase (IPr) staining in cell culture were conducted for detection of BHV-1 in suspected cattle, buffalo coats and sera from different localities in Egypt (El-Sharquia, Dumyat and El-Fayoum). A total of 15/93 (16.1%) of cattle, 4/55 (7.3%) of buffalo and 0/31 (0%) of sheep clinical specimens were BHV-1 positive in this study. Nested-PCR was superior to immunodetection using IFA and IPr after VI procedure. Besides, it was the most discriminate assay that detected BHV-1 viral DNA extracted directly extracted directly from cattle and buffalo clinical specimens (100%). While, none of the sheep viral isolates (0/3 ; 0%) could be identified as BHV-1 by neted-PCR, although 2/3 of these isolates (from nasal swabs) tested positive by IFA, IPr and the first round PCR. Also, a buffy coat buffalo viral isolate tested positive with the first round PCR while , it turned repeatedly negative to VI, IFA, IPr and nested-PCR testing. These nested-PCR testing. These nested-PCR negative viral isolates were regarded as antigenically related , yet genetically distinct, other ruminant herpes viruses that require further studies. Finding of this study emphasized the preponderant utility of the BHV-1 gB based nested PCR as a sensitive, discriminative and rapid tool for epidemiological studies and control programs of BHV-1 infections, without confusion with other ruminant herpesviruses. Accordingly, it is recommended for routine screening of not only local but also imported animals and biologics for BHV-1 contamination.
Publication year 2005
Pages 125-136
Availability location القاهرة - ش السكة البيضاء - العباسية
Availability number
Organization Name
City Cairo
serial title THE EGYPTIAN JOURNAL OF IMMUNOLOGY
ISSN 1110-4902
Author(s) from ARC
Agris Categories Animal diseases
Proposed Agrovoc bovine herpesvirus-1;molecular detection;
Publication Type Journal
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